J. Ashot Kozak, Ph.D.

NeuroScience Cell Bio Physio-COSM
Associate Professor, Neuroscience Cell Biology & Physiology
Biological Sciences Bldg II 251A, 3640 Colonel Glenn Hwy, Dayton, OH 45435-0001

Director, NCBP Seminars Program

Education History

Ph.D.: Icahn School of Medicine at Mount Sinai, New York City
Postdoctoral:    HHMI at Mt Sinai (Neuroscience); 

                            University of California, Irvine (Physiology & BIophysics)



Human Physiology (BMS 8620) Co-Director: Immunity, Blood, Molecular Endocrinology, Calcium regulation
Neuroscience and Physiology (BMS 8750): Pain and Ion Channels, Control of Intracellular Calcium
Endocrine (SMD 561): Physiology of Parathyroid Glands
Ion Channels (BMS 8530): TRP channels; 

Physiology Seminar (PN 7900), Anatomy Seminar (ANT 7900), Anatomy & Physiology Seminar (BMS 9900), Director

Research Interests

Cation channels expressed in leukocytes; Calcium entry channels; Magnesium deficiency; Transient Receptor Potential (TRP) channels; Ion channels involved in nociception.


National Institute of Allergy and Infectious Diseases (NIAID)


Director, NCBP Departmental Seminar Series

Member, CoSM IT Committee

Member, BSoM Research Committee

Member, BMS PhD Program Nominating Committee

Faculty Advisor, BMSSA (BMS PhD Student Association)

Supervised Student Research

Pavani Beesetty, BMS PhD Program

Jananie Rockwood, BMS PhD Program

Charles Tuan Luu, Neurosci. and Physiol. Master's Program

Ngocminh Truong, Honors Undergraduate Program

Alayna Mellott, Neurosci. and Physiol. Master's Program

Naga Lakshmi Gandi, Microbiology and Immunology Master's Program

Katherine Holderby, Physiology and Neuroscience Undergraduate Program



Holderby, K., Kozak, J.A. (2024) USING TEA AND TRIS UPTAKE FOR BLOCKADE OF TRPM7 CHANNELS IN INTACT CELLS. Biophysical Society 68th Annual Meeting Abstracts, Volume 123, Issue 3, Supplement 1

Holderby, K., Kozak, J.A. (2024) Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.  Frontiers in Pharmacology, Volume 15 https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar...

Kozak, J.A. (2022) Suppression of store-operated calcium entry channels and cytokine release by cannabinoids.   Function (Oxford) https://doi.org/10.1093/function/zqac044

Sun, L., Ekkati, A., Liardo, E., Zhelay, T., Kozak, J.A. (2022) Discovery of indole-3-carboxamides as fast onset and reversible Orai channel blockers: Structure-activity relationship and inhibition of T cell activation. ACS Spring 2022, San Diego

Chokshi, R., Bennett, O., Zhelay, T., Kozak, J.A. (2021)  NSAIDs naproxen, ibuprofen, salicylate and aspirin inhibit TRPM7 channels by cytosolic acidification. Frontiers in Physiology  https://www.frontiersin.org/articles/10.3389/fphys.2021.727549/abstract

Beesetty, P., Rockwood, J., Kaitsuka, T., Zhelay, T., Hourani, S., Matsushita, M., Kozak, J.A. (2021) Phagocytic activity of splenic macrophages is enhanced and accompanied by cytosolic alkalinization in TRPM7 kinase-dead mice. FEBS Journal 288(11):3585-3601. Epub 2021 Jan 6. https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.15683

Mellott, A., Rockwood, J., Zhelay, T., Luu, C.T., Kaitsuka, T., Kozak, J.A. (2020) TRPM7 channel activity in Jurkat T lymphocytes during  magnesium depletion and loading: implications for divalent metal entry and cytotoxicity. Pflügers Archiv European Journal of Physiology 472:1589–1606 https://rdcu.be/b7z3d

Zhelay, T., Wieczerzak, K.B., Beesetty, P., Alter, G.M., Matsushita, M., Kozak, J.A. (2018) Depletion of plasma membrane-associated phosphoinositides mimics inhibition of TRPM7 channels by cytosolic Mg2+, spermine and pH.  J. Biological Chemistry 293(47):18151-18167 http://www.jbc.org/content/293/47/18151.full?sid=e84ea1e1-05b9-4a1c-b544...

Beesetty, P., Wieczerzak, K.B., Gibson, J. N., Kaitsuka, T., Luu, C.T., Matsushita, M., Kozak, J.A. (2018) Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry. Scientific Reports 8:3023  http://rdcu.be/GWCR

Kozak, J. A. and Rychkov, G. (2017) Electrophysiological methods for recording CRAC and TRPV5/6 channels. Calcium entry channels in non-excitable cells. Edited by Kozak, J. A. and Putney, J.W. Jr., Boca Raton, CRC Press    https://www.ncbi.nlm.nih.gov/books/NBK531438/

Kursan, S., McMillen, T.S., Beesetty, P., Diaz-Junior, E., Almutairi, M.M., Sajib, A.A., Kozak, J.A., Aguilar-Bryan, L., Di Fulvio, M. (2017) The neuronal K+Cl- co-transporter 2 (Slc12a5) modulates insulin secretion. Scientific Reports (Nature publishing group) 7:1732. http://rdcu.be/rWmg

GIbson, J. N, Beesetty, P., Sulentic, C., Kozak, J.A. (2016) Rapid quantification of mitogen-induced blastogenesis in T lymphocytes for identifying immunomodulatory drugs. JoVE, www.jove.com/video/55212/rapid-quantification-mitogen-induced-blastogene...

Kaitsuka, T.,  Katagiri, C., Beesetty, P., Nakamura, K., Hourani, S., Tomizawa, K.,  Kozak, J.A., Matsushita, M. (2014) Inactivation of TRPM7 kinase activity does not impair its channel function in mice. Scientific Reports (Nature publishing group) 4: 5718.

Chokshi R, et al. (2012) 2-Aminoethyl diphenyl borinate (2-APB) inhibits TRPM7 channels through an intracellular acidification mechanism. Channels (Austin) 6 (5): 362-369.

Chokshi, R. H., Matsushita, M., Kozak, J.A. (2012) Sensitivity of TRPM7 channels to Mg2+ characterized in cell-free patches of Jurkat T lymphocytes. Am. J. Physiol. Cell Physiol. 302: C1642-1651.

Chokshi, R. H., Matsushita, M., Kozak, J.A. (2012) Detailed examination of Mg2+ and pH sensitivity of human TRPM7 channels. Am. J. Physiol. Cell Physiol. 302(7): C1004-C1011.

Lioudyno, M.I., Broccio, M., Sokolov, Y., Rasool, S., Wu, J., Alkire, M.T., Liu, V., Kozak, J.A., Dennison, P.R., Glabe, C.G., Lösche, M., Hall, J.E. (2012) Effect of synthetic Aβ peptide oligomers and fluorinated solvents on Kv1.3 channel properties and membrane conductance. PLoS One 7(4): e35090.

Zhang S.L.*Kozak J.A.*, Jiang W., Yu Y., Penna, A., Chi V., Cahalan, M.D. (2008) Store-dependent and -independent modes regulating Ca2+ Release-Activated Ca2+ channel activity of human Orai1 and Orai3. J. Biol. Chem. 283:17662-17671. *equal contributors

Lioudyno M.I., Kozak, J.A., Penna A., Safrina O., Zhang S.L., Roos J., Stauderman K.A., Cahalan M.D. (2008) Orai1 and STIM1 move to the immunological synapse and are up-regulated during T cell activation. Proc. Natl. Acad. Sci. USA 105(6):2011.

Jovanovska, A., Karczewski, J., Cook, S. and Kozak J.A. (2007) Regulation of proton-activated ASIC1 channels by extracellular organic cations. Society for Neurosci. Annual Meeting Abstract.

Sokolov Y., Kozak J.A., Kayed, R., Chanturiya A., Glabe, C., Hall, J.E. (2006) Soluble amyloid oligomers increase bilayer conductance by altering dielectric structure. J. General Physiol. 128:637.

Zhang, S.L., Yu, Y., Roos, J., Kozak, J.A., Deerinck, T.J., Ellisman, M.H., Stauderman, K.A., Cahalan, M.D.(2005) STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature 437:902.

Kozak, J.A., Matsushita, M., Nairn, A.C., Cahalan, M.D. (2005) Charge screening by internal pH and polyvalent cations as a mechanism for activation, inhibition and rundown of TRPM7/MIC channels. J. General Physiol.126(5):499.

Matsushita M., Kozak J.A., Shimizu Y., McLachlin D.T., Yamaguchi H., Wei F.Y., Tomizawa K., Matsui H., Chait B.T., Cahalan M.D., Nairn A.C. (2005) Channel function is dissociated from the intrinsic kinase activity and autophosphorylation of TRPM7/ChaK1. J. Biol. Chem. 280(21):20793.

Kozak J.A., Cahalan M.D. (2003) MIC channels are inhibited by internal divalent cations but not ATP.Biophysical Journal 84(2 Pt 1):922.

Kozak J.A. et al. (2002) Distinct Properties of CRAC and MIC Channels in RBL Cells. J. General Physiol.120(2):221.

Perez C.A., Huang L., Rong M., Kozak J.A., Preuss A.K., Zhang H., Max M., Margolskee R.F. (2002) A transient receptor potential channel expressed in taste receptor cells. Nature Neuroscience 5(11):1169.

Kozak J.A.Misler, S., Logothetis, D.E. (1998) Characterization of a Ca2+-activated K+ current in insulin-secreting murine βTC-3 cells. Journal of Physiology 509 (Pt. 2):355.

Kozak J. A., Logothetis D.E. (1997) A calcium-dependent chloride current in insulin-secreting βTC-3 cells.  Pflügers Archiv European Journal of Physiology 433:679.      

Professional Affiliations/Memberships

Biophysical Society

American Physiological Society

Ohio Physiological Society

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