J. Ashot Kozak, Ph.D.

NeuroScience Cell Bio Physio-COSM
Associate Professor, Neuroscience Cell Biology & Physiology
Biological Sciences Bldg II 251A, 3640 Colonel Glenn Hwy, Dayton, OH 45435-0001

Director, NCBP Seminars Program

Education History: 

Ph.D.: Mount Sinai School of Medicine, New York City, 1998
Postdoctoral: University of California at Irvine



Human Physiology (BMS 8620): Immunity, Blood, Molecular Endocrinology
Neuroscience and Physiology (BMS 8750): Pain and Ion Channels, Control of Intracellular Calcium
Endocrine (SMD 561): Physiology of Parathyroid Glands
Ion Channels (BMS 8530): TRP channels;  Physiology Seminar (P&N 8000)


Research statement: 

Cation channels expressed in leukocytes; calcium entry channels; Transient Receptor Potential (TRP) channels; ion channels involved in nociception.


National Institute of Allergy and Infectious Diseases (NIAID)


Director, NCBP Departmental Seminar Series

Students Advised: 

Pavani Beesetty, BMS PhD Program

Jananie Rockwood, BMS PhD Program

Charles Tuan Luu, Neurosci. and Physiol. Master's Program

Ngocminh Truong, Honors undergraduate program

Alayna Mellott, Neurosci. and Physiol. Master's Program




  Mellott, A., Rockwood, J., Zhelay, T., Luu, C.T., Kozak, J.A. (under review 6/2020) TRPM7 channel activity in Jurkat T lymphocytes during  magnesium depletion and loading: implications for divalent metal entry and cytotoxicity.

  Beesetty, P., Rockwood, J., Kaitsuka, T., Zhelay, T., Matsushita, M., Kozak, J.A. (under review 6/2020) Phagocytic activity in macrophages ioslated from TRPM7 kinase-dead mice.

Zhelay, T., Wieczerzak, K.B., Beesetty, P., Alter, G.M., Matsushita, M., Kozak, J.A. (2018) Depletion of plasma membrane-associated phosphoinositides mimics inhibition of TRPM7 channels by cytosolic Mg2+, spermine and pH.  J. Biological Chemistry 293(47):18151-18167 http://www.jbc.org/content/293/47/18151.full?sid=e84ea1e1-05b9-4a1c-b544...

Beesetty, P., Wieczerzak, K.B., Gibson, J. N., Kaitsuka, T., Luu, C.T., Matsushita, M., Kozak, J.A. (2018) Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry. Scientific Reports 8:3023  http://rdcu.be/GWCR

Kozak, J. A. and Rychkov, G. (2017) Electrophysiological methods for recording CRAC and TRPV5/6 channels. Calcium entry channels in non-excitable cells. Edited by Kozak, J. A. and Putney, J.W. Jr., Boca Raton, CRC Press    https://www.ncbi.nlm.nih.gov/books/NBK531438/

Kursan, S., McMillen, T.S., Beesetty, P., Diaz-Junior, E., Almutairi, M.M., Sajib, A.A., Kozak, J.A., Aguilar-Bryan, L., Di Fulvio, M. (2017) The neuronal K+Cl- co-transporter 2 (Slc12a5) modulates insulin secretion. Scientific Reports (Nature publishing group) 7:1732. http://rdcu.be/rWmg

GIbson, J. N, Beesetty, P., Sulentic, C., Kozak, J.A. (2016) Rapid quantification of mitogen-induced blastogenesis in T lymphocytes for identifying immunomodulatory drugs. JoVE, www.jove.com/video/55212/rapid-quantification-mitogen-induced-blastogene...

Kaitsuka, T.,  Katagiri, C., Beesetty, P., Nakamura, K., Hourani, S., Tomizawa, K.,  Kozak, J.A., Matsushita, M. (2014) Inactivation of TRPM7 kinase activity does not impair its channel function in mice. Scientific Reports (Nature publishing group) 4: 5718.

Chokshi R, et al. (2012) 2-Aminoethyl diphenyl borinate (2-APB) inhibits TRPM7 channels through an intracellular acidification mechanism. Channels (Austin) 6 (5): 362-369.

Chokshi, R. H., Matsushita, M., Kozak, J.A. (2012) Sensitivity of TRPM7 channels to Mg2+ characterized in cell-free patches of Jurkat T lymphocytes. Am. J. Physiol. Cell Physiol. 302: C1642-1651.

Chokshi, R. H., Matsushita, M., Kozak, J.A. (2012) Detailed examination of Mg2+ and pH sensitivity of human TRPM7 channels. Am. J. Physiol. Cell Physiol. 302(7): C1004-C1011.

Lioudyno, M.I., Broccio, M., Sokolov, Y., Rasool, S., Wu, J., Alkire, M.T., Liu, V., Kozak, J.A., Dennison, P.R., Glabe, C.G., Lösche, M., Hall, J.E. (2012) Effect of synthetic Aβ peptide oligomers and fluorinated solvents on Kv1.3 channel properties and membrane conductance. PLoS One 7(4): e35090.

Zhang S.L.*Kozak J.A.*, Jiang W., Yu Y., Penna, A., Chi V., Cahalan, M.D. (2008) Store-dependent and -independent modes regulating Ca2+ Release-Activated Ca2+ channel activity of human Orai1 and Orai3. J. Biol. Chem. 283:17662-17671. *equal contributors

Lioudyno M.I., Kozak, J.A., Penna A., Safrina O., Zhang S.L., Roos J., Stauderman K.A., Cahalan M.D. (2008) Orai1 and STIM1 move to the immunological synapse and are up-regulated during T cell activation. Proc. Natl. Acad. Sci. USA 105(6):2011.

Jovanovska, A., Karczewski, J., Cook, S. and Kozak J.A. (2007) Regulation of proton-activated ASIC1 channels by extracellular organic cations. Society for Neurosci. Annual Meeting Abstract.

Sokolov Y., Kozak J.A., Kayed, R., Chanturiya A., Glabe, C., Hall, J.E. (2006) Soluble amyloid oligomers increase bilayer conductance by altering dielectric structure. J. General Physiol. 128:637.

Zhang, S.L., Yu, Y., Roos, J., Kozak, J.A., Deerinck, T.J., Ellisman, M.H., Stauderman, K.A., Cahalan, M.D.(2005) STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature 437:902.

Kozak, J.A., Matsushita, M., Nairn, A.C., Cahalan, M.D. (2005) Charge screening by internal pH and polyvalent cations as a mechanism for activation, inhibition and rundown of TRPM7/MIC channels. J. General Physiol.126(5):499.

Matsushita M., Kozak J.A., Shimizu Y., McLachlin D.T., Yamaguchi H., Wei F.Y., Tomizawa K., Matsui H., Chait B.T., Cahalan M.D., Nairn A.C. (2005) Channel function is dissociated from the intrinsic kinase activity and autophosphorylation of TRPM7/ChaK1. J. Biol. Chem. 280(21):20793.

Kozak J.A., Cahalan M.D. (2003) MIC channels are inhibited by internal divalent cations but not ATP.Biophysical Journal 84(2 Pt 1):922.

Kozak J.A. et al. (2002) Distinct Properties of CRAC and MIC Channels in RBL Cells. J. General Physiol.120(2):221.

Perez C.A., Huang L., Rong M., Kozak J.A., Preuss A.K., Zhang H., Max M., Margolskee R.F. (2002) A transient receptor potential channel expressed in taste receptor cells. Nature Neuroscience 5(11):1169.

Kozak J.A.Misler, S., Logothetis, D.E. (1998) Characterization of a Ca2+-activated K+ current in insulin-secreting murine βTC-3 cells. Journal of Physiology 509 (Pt. 2):355.

Professional Affiliations/Memberships: 

Biophysical Society

American Physiological Society

Is this you? Log in to update your profile.