Quan Zhong, Ph.D.

Department:
Biological Sciences
Title:
Associate Professor
Address:
Diggs Laboratory 254, 3640 Colonel Glenn Hwy, Dayton, OH 45435-0001

Education History

2006-2013   Postdoc, Systems Biology, Harvard Medical School (Advisor: Dr. Marc Vidal)
1999-2005   Ph.D., Genetics, Wayne State University (Advisor: Dr. Miriam Greenberg)
1993-1997   B.S., Microbiology and Microbiological Engineering, Fudan University

Teaching

Undergraduate Lecture course
•    BIO 2110  Principles of Molecular and Classical Genetics
•    BIO 4020  Current Literature in Network Biology
•    BIO 4020  Current Literature in Genetics and Genomics
Graduate Lecture course
•    BIO 6020  Current Literature in Network Biology
•    BIO 6020  Current Literature in Genetics and Genomics
•    BIO 8000  Graduate Seminar

Research Statement

The Zhong Lab uses genetic and cell biology approaches to gain mechanistic understanding of how several neurodegenerative disease-associated proteins disrupt highly conserved cellular processes causing loss of cell integrity and cell death. We work primarily with the budding yeast, Saccharomyces cerevisiae. This single-cell organism is a classical model and one of the best characterized eukaryotes. Owning to the availability of genome-scale genetic tools, S. cerevisiae has become a fascinating system for functional genomics and systems biology research. Many cellular processes are well conserved from yeast to human, making it a viable model for studying human disease. 

One focus of the lab is a protein called α-synuclein, a small lipid-binding protein enriched in the brain. Insoluble misfolded α-synuclein is the primary component of Lewy body, the pathological hallmark of Parkinson's disease. Lewy body pathology is also frequently found in Lewy body dementia and Alzheimer’s disease. Decades after the first discovery of α-synuclein in the Lewy body and mutations in the human gene encoding α-synuclein as genetic causes to Parkinson disease, the cellular function of α-synuclein remains obscure. Aberrant accumulation of α-synuclein induces cellular toxicity in diverse model organisms, including the budding yeast. How such processes are regulated remains unclear. Using high-throughput genetic screens, our lab has identified a network of human genes that promote or reduce the toxicity of α-synuclein. Our current focus is to study the modifier mechanisms underlying each identified toxicity enhancer or suppressor. Our long-term goal is to understand the role of α-synuclein in normal cellular functions and in disease from a global perspective (Funding: NIH/NIA,1R21AG067446-01).

Students Advised

We welcome both undergraduate and graduate students to join our research team. We seek to recruit people with diverse background and experience. We believe that diversity helps us to grow, to create new ideas and to excel. We are committed to maintain a safe and supportive environment for everyone in the group. We value professionalism in science and continue to make every effort to be transparent in every aspect of our work. We strive to attain equity in research by promoting a respectful lab environment and by fostering open and honest communications. Please feel free to reach out to Dr. Zhong to talk about your career goals and research interest. We look forward to working with new people. Below are the past and current members of the Zhong lab.

Undergraduate students from Wright State University
Annabel Almazan (Honors, Biological Sciences)
Roselle Almazan (Honors, Biological Sciences)
Haley Curry (Biological Sciences)    
Ellie Fisk (Biological Sciences)    
Breonna Gillespie (Biological Sciences)
Kyle Hazlett (Biological Sciences)    
Cameron Moon (Biological Sciences)    
Pooja Shirahatti (Biological Sciences)    
Daniel Smith (Biological Sciences)    
Catherine Thomas (Neuroscience)
Huijuanz Zhu (Medical Laboratory Science)

Undergraduate students from other institutions
Alexis Barfield (Elizabeth City State University)
Anna Heinrich (University of Connecticut)
Yun-Kyung Kim (Brandeis University)
Amoni Tyler (Central State University)

Graduate students
Annabel Almazan (M.S. program in Biology)
Angela Braunschweiger (M.S. program in Biology)
Ishita Haider (Ph.D. program in Biomedical Sciences)
Md Moydul Islam (M.S. program in Biology)
Rajalakshmi Santhanakrishnan (Ph.D. program in Biomedical Sciences)

High-school students
Kim Hyemin (Centerville High School)
Cameron Croake-Uleman (The Dayton Regional STEM School)

Research Associate
Annabel V. Almazan (M.S.)
Shuzhen Chen (M.D.)
Yali Chi (Ph.D.)
Vaishnavi Ragavapuram (M.S.)

Publications

Kim JH, Seo Y, Jo M, Jeon H, Kim, YS, Kim EJ, Seo D, Lee WH, Kim SR, Yachie N, Zhong Q, Vidal M, Roth FP, Suk K. A genetic interaction screen in yeast identifies genes relevant to human PAK1 function in glioma. Journal of Biolgical Chemistry 2020, 295:16906-16919  

Hayden E*, Chen S*, Chumley A, Xia C, Zhong Q$, Ju S$. A genetic screen for human genes suppressing FUS induced toxicity in yeast. G3 Genes Genomes Genetics 2020, 10:1843. *Equal contributors $Co-corresponding  

Kim JH, Seo Y, Jo M, Jeon H, Lee WH, Yachie N, Zhong Q, Vidal M, Roth FP, Suk K. Yeast-based genetic interaction analysis of human kinome. Cells 2020, 9:1156.

Luck K, Kim DK, Lambourne L, Spirohn K, Begg BE, Bian W, Brignall R, Cafarelli T, Campos-Laborie FJ, Charloteaux B, Choi D, Coté AG, Daley M, Deimling S, Desbuleux A, Dricot A, Gebbia M, Hardy MF, Kishore N, Knapp JJ, Kovács IA, Lemmens I, Mee MW, Mellor JC, Pollis C, Pons C, Richardson AD, Schlabach S, Teeking B, Yadav A, Babor M, Balcha D, Basha O, Bowman-Colin C, Chin SF, Choi SG, Colabella C, Coppin G, D'Amata C, De Ridder D, De Rouck S, Duran-Frigola M, Ennajdaoui H, Goebels F, Goehring L, Gopal A, Haddad G, Hatchi E, Helmy M, Jacob Y, Kassa Y, Landini S, Li R, van Lieshout N, MacWilliams A, Markey D, Paulson JN, Rangarajan S, Rasla J, Rayhan A, Rolland T, San-Miguel A, Shen Y, Sheykhkarimli D, Sheynkman GM, Simonovsky E, Taşan M, Tejeda A, Tropepe V, Twizere JC, Wang Y, Weatheritt RJ, Weile J, Xia Y, Yang X, Yeger-Lotem E, Zhong Q, Aloy P, Bader GD, De Las Rivas J, Gaudet S, Hao T, Rak J, Tavernier J, Hill DE, Vidal M, Roth FP, Calderwood MA. A reference map of the human binary protein interactomeNature 2020 580:402-408. doi: 10.1038/s41586-020-2188-x.

Hayden E, Chen S, Chumley A, Zhong Q, Ju S. Mating-based overexpression library screening in yeast. Journal of Visualized Experiments 137 (2018) doi: 10.3791/57978.

Khurana V*, Peng J*, Chung CY*, Auluck PK, Fanning S, Tardiff DF, Bartels T, Koeva M, Eichhorn SW, Benyamini H, Lou Y, Nutter-Upham A, Baru V, Freyzon Y, Tuncbag N, Costanzo M, SanLuis BJ, Schöndorf DC, Barrasa MI, Ehsani S, Sanjana N, Zhong Q, Gasser T, Bartel DP, Vidal M, Deleidi M, Boone C, Fraenkel E, Berger B, Lindquist S.   Genome-scale networks link neurodegenerative disease genes to alpha-synuclein through specific molecular pathways. Cell Systems 4:157 (2017) *Equal contributors

Zhong Q*$, Pevzner SJ*, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Taşan M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barabási AL, Hill DE, Aloy P, Cusick ME, Xia Y$, Roth FP$, Vidal M$. An inter-species protein-protein interaction network across vast evolutionary distance. Molecular Systems Biology 12:865 (2016) *Equal contributors $Co-corresponding

Yang X*, Coulombe-Huntington J*, Kang S*, Sheynkman GM*, Hao T*, Richardson A, Sun S, Yang F, Shen Y, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M. Widespread expansion of protein interaction capabilities by alternative splicing. Cell 164:805 (2016) *Equal contributors

Sahni N*, Yi S*, Taipale M*, Fuxman Bass JI*, Coulombe-Huntington J,* Yang F, Peng J, Weile J, Karras GI, Wang Y, Kovács IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y1, Cusick ME, Salehi-Ashtiani K8, Whitesell LJ, Sunyaev S, Berger B, Barabási AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJ, Lindquist S, Vidal M. Widespread macromolecular interaction perturbations in human genetic disorders. Cell 161:647 (2015) *Equal contributors

Rolland T*, Taşan M*, Charloteaux B*, Pevzner SJ*, Zhong Q*, Sahni N*, Yi S*, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabási AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP, Vidal M. A proteome-scale map of the human interactome network. Cell 159:1212 (2014) *Equal contributors



Wang W, Zhong Q, Teng L, Bhatnagar N, Sharma B, Zhang X, Luther W 2nd, Haynes LP, Burgoyne RD, Vidal M, Volchenboum S, Hill DE, George RE. Mutations that disrupt PHOXB interaction with the neuronal calcium sensor HPCAL1 impede cellular differentiation in neuroblastoma. Oncogene 33:3316 (2014)

Sahni N, Yi S, Zhong Q, Jailkhani N, Charloteaux B, Cusick ME and Vidal M. Edgotype: a fundamental link between genotype and phenotype. Curr Opin Genet Dev. 23:649 (2013) 



Lambert JP, Ivosev G, Gouzens AL, Larsen B, Taipale M, Lin ZY, Zhong Q, Lindquist S, Vidal M, Aebersold R, Pawson T, Bonner R, Tate S, Gingras AC. Mapping differential interactomes by affinity purification coupled with data-independent mass spectrometry acquisition.  Nat Methods 10:1239 (2013)

Ju S, Tardiff DF, Han H, Divya K, Zhong Q, Maquat LE, Bosco DA, Hayward LJ, Brown RH Jr, Lindquist S, Ringe D, Petsko GA. A yeast model of FUS/TLS-dependent cytotoxicity. J  PLoS Biol 9:e1001052 (2011)



Charloteaux B*, Zhong Q*, Dreze M*, Cusick ME, Hill DE, Vidal M. Protein-protein interaction networks: Forward and reverse edgetics. Methods Mol Biol 759:197 (2011) *Equal contributors


Zhong Q*, Simonis N*, Li QR*, Charloteaux B*, Heuze F*, Klitgord N*, Tam S, Yu H, Venkatesan K, Mou D, Swearingen V, Yildirim MA, Yan H, Dricot A, Szeto D, Lin C, Hao T, Fan C, Milstein S, Dupuy D, Brasseur R, Hill DE, Cusick ME, Vidal M. Edgetic perturbation models of human inherited disorders. Mol Syst Biol 5:321. (2009) *Equal contributors
 [Highlighted in Mol Syst Biol 5:322 (2009)
; Nat Rev Genet 11:4-5 (2010)
; Nature 463:405 (2010)
]


Dreze M*, Charloteaux B*, Milstein S*, Vidalain PO*, Yildirim MA, Zhong Q, Svrzikapa N, Romero V, Laloux G, Brasseur R, Vandenhaute J, Boxem M, Cusick ME, Hill DE, Vidal M. ‘Edgetic’ perturbation of a C. elegans BCL2 ortholog.  Nat Methods 6:843-9. (2009) *Equal contributors
 [Highlighted in Nat Methods 6:797-8 (2009)

]

De Nicolo A, Parisini E*, Zhong Q*, Dalla Palma M, Stoeckert KA, Domchek SM, Nathanson KL, Caligo MA, Vidal M, Cusick ME, Garber JE. Multimodal assessment of protein functional deficiency supports pathogenicity of BRCA1. Cancer Res 69:7030 (2009) *Equal contributors


Zhou J*, Zhong Q*, Li G, Greenberg ML. Loss of cardiolipin leads to longevity defects that are alleviated by alterations in stress response signaling.J Biol Chem 284:18106 (2009) *Equal contributors


Li QR*, Carvunis AR*, Yu H*, Han JD*, Zhong Q, Simonis N, Tam S, Hao T, Klitgord NJ, Dupuy D, Mou D, Wapinski I, Regev A, Hill DE, Cusick ME, Vidal M. Revisiting the Saccharomyces cerevisiae predicted ORFeome. Genome Res 18:1294. (2008) *Equal contributors

Zhong Q*, Li G*, Gvozdenovic-Jeremic J, Greenberg ML. Up-regulation of the cell integrity pathway in Saccharomyces cerevisiae suppresses temperature sensitivity of the pgs1Δ mutant.  J Biol Chem 282:15946. (2007) *Equal contributors

Zhong Q, Greenberg ML. Deficiency in mitochondrial anionic lipids synthesis impairs cell wall biogenesis. Biochem Soc Trans 33:1158. Review. (2005)



Zhong Q*, Gvozdenovic-Jeremic J*, Webster P, Zhou J, Greenberg ML. Loss of function of KRE5 suppresses temperature sensitivity of mutants lacking mitochondrial anionic lipids.   Mol Biol Cell 16:665-75. (2005) *Equal contributors

Zhong Q* Gohil VM* Ma L* Greenberg ML. Absence of cardiolipin results in temperature sensitivity, respiratory defects, and mitochondrial DNA instability independent of pet56 .J Biol Chem 279:32294. (2004) *Equal contributors

Zhong Q, Greenberg ML. Regulation of phosphatidylglycerophosphate synthase by inositol in Saccharomyces cerevisiae is not at the level of PGS1 mRNA abundance.   J Biol Chem 278:33978. (2003)

Gu Z*, Gohil V*, Zhong Q*, Schlame M and Greenberg ML. The biosynthesis and remodeling of cardiolipin in LIPIDS: Glycerolipid Metabolizing Enzymes. (Haldar, D. and Das, S.K., ed.) pp 67-84. Review. (2002) *Equal contributors

Cui JP, Zhong Q, Li WZ, Yan J, Shen DL. Factors affecting population biology of Wolbachia in the small brown planthoppers, Laodelphax striatellus (Homoptera: Delphacidae).  J Fudan Univ: Nat Sci 37:542 (1998)

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