Sean William Harshman, PhD

Adjunct Assistant Professor, Biochemistry & Molecular Biology
Area B, 2510 5th St Bldg 840, Wright Patterson AFB, OH 45433-7951

Education History

Postdoc/Research Scientist, 2014 - Air Force Research Laboratory, UES Inc.

Ph.D., 2013 - Cancer Biology, Immunology, The Ohio State University

M.S., 2009 - Pharmacology/Toxicology,  Wright State University

B.S., 2005 – Chemistry,  Wilmington College

Research Statement

Dr. Sean Harshman currently serves as a research scientist at the Air Force Research Laboratory (AFRL) conducting studies to determine the molecular signatures of human performance and physiology using high end mass spectrometry. His work utilizes non-invasive media sources, such as exhaled breath and sweat, to identify potential volatile, metabolite, protein and lipid biomarkers of hypoxia, stress and fatigue.


27. Harshman SW, Browder AB, Davidson CN, Pitsch RL, Strayer KE, Schaeublin NM, Phelps MS, O’Connor ML, Mackowski NS, Barrett KN, Eckerle JJ, Strang AJ, Martin JA. The Impact of Nutritional Supplementation on Sweat Metabolomic Content: A Proof-of-Concept Study. Front. Chem. 2021;9:659583.

26. Harshman SW, Strayer KE, Davidson CN, Pitsch RL, Narayanan L, Scott AM, Schaeublin NM, Wiens TL, Phelps MS, O’Connor ML, Mackowski NS, Barrett KN, Leyh SM, Eckerle JJ, Strang AJ, Martin JA. Rate Normalization for Sweat Metabolomic Biomarker Discovery. Talanta 2021;223(1):121797.

25. Soliman SHA, Stark AE, Gardner ML, Harshman SW, Breece CC, Amari F, Orlaccio A, Chen M, Tessari A, Martin JA, Visone R, Freitas MA, La Perle KMD, Palmieri D, Coppola V. Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. Sci. Rep. 2020;10:7138.

24. Harshman SW, Pitsch RL, Davidson CN, Lee EM, Scott AM, Hill EM, Mainali P, Brooks ZE, Strayer KE, Schaeublin NM, Wiens TL, Brothers MC, Drummond LA, Martin JA. Evaluation of a Standardized Collection Device for Exhaled Breath Sampling onto Thermal Desorption Tubes. J. Breath Res. Breath Res. 2020;14(3):036004. 

23. Harshman SW, Pitsch RL, Davidson CN, Scott AM, Hill EM, Smith ZK, Strayer KE, Schaeublin NM, Wiens TL, Brothers MC, Slusher GM, Steele ML, Geier BA, Fan M, Drummond LA, Martin JA. Characterization of Standardized Breath Sample for Off-Line Field Use. J. Breath Res. 2020;14(1):016009.

22. Jung S, Hatcher-Solis C, Moore R, Bechmann N, Harshman S, Martin J, Jankord R. Noninvasive brain stimulation enhances memory acquisition and is associated with synaptoneurosome modification in the rat. eNeuro2019;6(6):0311-19.

21. Harshman SW, Pitsch RL, Schaeublin NM, Smith ZK, Strayer KE, Phelps MS, Qualley AV, Cowan DW, Rose SD, O’Connor ML, Eckerle JJ, Das T, Barbey AK, Strang AJ, Martin JA. Metabolomic stability of exercise-induced sweat. J. Chrom. B 2019;1126-1127:121763.

20. Harshman SW, Pitsch RL, Smith ZK, O’Connor ML, Geier BA, Qualley, AV, Schaeublin, NM, Fischer MV, Eckerle, JJ, Strang AJ, Martin JA. The proteomic and metabolomic characterization of exercise-induced sweat for human performance monitoring: a pilot investigation. PLOS ONE 2018;13(11): e0203133.

19. Harshman SW, Geier BA, Qualley AV, Drummond LA, Flory LE, et al. Exhaled Isoprene for Monitoring Recovery from Acute Hypoxic Stress. J. Breath Res. 2017;11(4):047111.

18. Harshman SW, Rubenstein MH, Qualley AV, Fan M, Geier BA, et al. Evaluation of Thermal Desorption Analysis on a Portable GC-MS System. Int. J. Env. Anal. Chem. 2017;97(3):247-263.

17. Harshman SW, Mani N, Geier, BA, Shepard P, Fan M, et al. Storage stability of exhaled breath on Tenax TA. J. Breath Res. 2016;10(4):046008.

16. Martin JA, Kwak J, Harshman SW, Chan K, Fan M, et al. Field Sampling Demonstration of Portable Thermal Desorption Collection and Analysis Instrumentation. Int. J. Env. Anal. Chem. 2016;1:1-21.

15. Canella A, Harshman SW, Freitas MA, Pichiorri F. The Potential Diagnostic Power of Extracellular Vesicle Analysis for Multiple Myeloma. Expert Rev. Mol. Diagn. 2016;16(3):277-284.

14. Harshman SW, Canella A, Ciarlariello PD, Branson OE, Agarwal K, et al. Proteomic Characterization of Circulating Extracellular Vesicles Identifies CD44 as a Novel Myeloma Associated Marker. J. Proteomics. 2015;136:89-98.

13. Harshman SW, Geier BA, Fan M, Rinehardt S, Watts BS, et al. The Identification of Hypoxia Biomarkers from Exhaled Breath under Normobaric Conditions. J. Breath Res. 2015;9(4):047103.

12. Harshman SW, Dershem VL, Fan M, Slusher GM, Flory LE, et al. The Stability of Tenax TA Thermal Desorption Tubes in Simulated Field Conditions on the HAPSITE® ER. Int. J. Env. Anal. Chem. 2015;95(11):1014-1029.

11.  Singh R, Harshman SW, Stark A, Mortazavi A, Lucus DM, et al. Proteomic Profiling Identifies Specific Histones Species Associated with Leukemic and Cancer Cells. Clin. Proteomics. 2015;12(1):22.

10. Renusch SR, Harshman SW, Pi H, Workman E, Wehr A, et al. Spinal Muscular Atrophy Biomarker Measurements from Blood Samples in a Clinical Trial of Valproic Acid in Ambulatory Adults. J. Neuromus. Dis. 2015;2:119-130.

9.   Kwak J, Fan M, Harshman SW, Garrison C, et al. Evaluation of Bio-VOC Sampler for Analysis of Volatile Organic Compounds in Exhaled Breath Samples. Metabolites. 2014;4(4):879-888.

8.   Harshman SW, Grigsby CC, Ott DK. Exhaled Breath Condensate for Proteomic Biomarker Discovery. Chromatography. 2014;1:108-119.

7.   Harshman SW, Hoover ME, Huang C, Branson OE, et al. Histone H1 Phosphorylation in Breast Cancer. J. Proteome Res. 2014;13(5):2453-2467.

6.   Choudhury M, Li H, Harshman S, Freitas M, Dugoff L. 8: Epigenetics and microRNA as a unifying mechanism in severe preeclampsia. Am. J. Obstet. Gynecol. 2014;210.

5.   Harshman SW, Chen MM, Branson OE, Jacob NK, et al. Characterization of linker histones across cellular compartments. J. Proteomics. 2013;91C:595-604.

4.   Harshman SW, Canella A, Ciarlariello PD, Rocci A, et al. Characterization of Multiple Myeloma Vesicles by Label-Free Relative Quantitation. Proteomics. 2013;13(20):3013-3029.

3.   Harshman SW, Young NL, Parthun MR, Freitas MA. Histone H1: Current Perspectives and Challenges. Nucleic Acids Res. 2013;41(21):9593-9609.

2.  Wang L, Harshman SW, Liu S, et al. Assaying pharmacodynamic endpoints with targeted therapy: flavopiridol and 17AAG induced phosphorylation of histone H1.5 in acute myeloid leukemia. Proteomics. 2010;10(23):4281–4292.

1. Goldcamp MJ, Underwood MN, Cloud JL, Harshman S, Ashley K. An Environmentally Friendly, Cost-Effective Determination of Lead in Environmental Samples Using Anodic Stripping Voltammetry. J. Chem. Educ. 2008;85(7):976.

Professional Affiliations/Memberships

American Society for Mass Spectrometry (ASMS)

International Association for Breath Research (IABR)

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