Mike Kemp, Ph.D.

Department:
Pharmacology & Toxicology-SOM
Title:
Assistant Professor, Pharmacology & Toxicology
Address:
Biological Sciences Bldg II 142, 3640 Colonel Glenn Hwy, Dayton, OH 45435-0001

 

Visit the Kemp Lab website to learn more about my research program.

For a short video overview of my research and teaching, click here.

 

Curriculum Vitae

Kemp_CV_031921.pdf 254.45 KB

Education History

EDUCATION

1999 B.S. in Biological Sciences,  Wright State University, Dayton, Ohio

2006 Ph.D. in Biomedical Sciences, Wright State University, Dayton, Ohio

 

POST-DOCTORAL TRAINING

2006-2011 DNA Repair and DNA Damage Signaling, University of North Carolina Department of Biochemistry & Biophysics, Chapel Hill, NC

2016-2017 Skin Biology and Aging, Wright State University Department of Pharmacology & Toxicology, Dayton, Ohio

Teaching

PTX-7002: Journal Club/Special Topics: Genome Stability and Human Disease (Fall, Spring, Summer)

PTX-8007: Career Planning in Pharmacology & Toxicology (Fall in-person; Spring online)

PTX-8020: Pharmacology &Toxicology of DNA Damaging Agents (Spring)

 

Research Statement

My research interests are in the area of the cellular DNA damage response, with a specific focus on DNA repair, DNA replication, and DNA damage kinase signaling in UV-irradiated human skin. In addition to finding new ways to prevent skin cancer, we are also interest in improving the effectiveness of cancer chemotherapy regimens involving DNA damaging agents through modification of DNA damage responses and the circadian clock.

My laboratory is currently funded by grants from the National Institute of General Medical Sciences and Ohio Cancer Research Associates.

A presentation of some of our recent work can be found here.

Service

Chair, Awards & Honors Committee, Environmental Mutagenesis and Genomics Society

Students Advised

I primarily train students in the Pharmacology and Toxicology Master's Degree Program and the Biomedical Sciences Ph.D. Program. Students interested in joining my lab should visit my website and contact me at mike.kemp@wright.edu. Graduate research assistantships are dependent upon available fundss.

Master of Science in Pharmacology and Toxicology students wishing to carry out thesis research in my laboratory are strongly encouraged to take PTX7002 (Journal Club: Genome Stability and Human Disease; Fall semseter) and PTX8020 (Pharmacology & Toxicology of DNA Damaging Agents; Spring semester), which provide background context and knowledge related to my area of research. Thus, students taking these courses will be better acquainted with my research program and will therefore be given preferrence over other students interested in joining my lab. For more information about my expectations for students working in my lab, see the "Expections for graduate students" file at the bottom of the screen.

Publications

Check out my publications on PubMed.

Original Research Articles:

Wright State University (2017-present)

  1. Hutcherson RJ, Gabbard RD, Castellanos AJ, Travers JB, and Kemp MG. (2021, in press). Age and insulin-like growth factor-1 impact PCNA mono-ubiquitination in UVB-irradiated human skin. J Biol Chem.
  2. Alkawar AMM, Castellanos AJ, Carpenter MA, Hutcherson RJ, Madkhali MAO, Johnson RM, Bottomley M, and Kemp MG. (2020). Insulin-like Growth Factor-1 Impacts p53 Target Gene Induction in UVB-irradiated Keratinocytes and Human Skin. Photochemistry & Photobiology. 96(6): 1332-1341. YouTube summary: click here.
  3. Choi J-H, Han S, and Kemp MG. (2020). Detection of the small oligonucleotide products of nucleotide excision repair in UVB-irradiated human skin. DNA Repair. 86: 102766. YouTube summary: click here.
  4. Travers JB, Kemp MG, Weir NM, Cates E, Alkawar AM, Majahan AS, Spandau DF. (2020). Wounding with a microneedling device corrects the inappropriate ultraviolet B radiation response in geriatric skin. Arch Dermatol Res. 312(1): 1-4.
  5. Shaj K, Hutcherson RJ, Kemp MG. (2020). ATR kinase activity limits mutagenesis and promotes the clonogenic survival of quiescent human keratinocytes exposed to UVB radiation. Photochemistry & Photobiology. 96(1): 105-112. YouTube summary: click here.
  6. Hutcherson RJ and Kemp MG. (2019). ATR kinase inhibition sensitizes quiescent human cells to the lethal effects of cisplatin but increases mutagenesis. Mutation Research. 816-818: 111678. YouTube summary: click here.
  7. Kemp MG, Krishnamurthy S, Kent MN, Schumacher DL, Sharma P, Excoffon KJDA, Travers JB. (2019). Spironolactone depletes the XPB protein and inhibits DNA damage responses in UVB-irradiated human skin. J Invest Dermatology.139(2): 448-454. YouTube summary: click here.
  8. Poudel S, Yao J, Kemp MG, Leffak M. (2018). Interaction between DUE-B and Treslin is required to load Cdc45 on chromatin in human cells. J Biol Chem. 293(37): 14497-14506.
  9. Dakup PP, Porter KI, Little AA, Gajula RP, Zhang H, Skorynakov E, Kemp MG, Van Dongen HPA, and Gaddameedhi S. (2018). The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models. Oncotarget. 9(18): 14524-14538.
  10. Baek S, Han S, Kang D, Kemp MG*, Choi JH. (2018). Simultaneous detection of nucleotide excision repair events and apoptosis-induced DNA fragmentation in genotoxin-treated cells. Scientific Reports. 8(2265): 1-11. (*co-corresponding author) YouTube summary: click here.
  11. Kemp MG. (2017). DNA damage-induced ATR kinase activation in non-replicating cells is regulated by the XPB subunit of transcription factor II-H (TFIIH). J Biol Chem. 292(30): 12424-12435.
  12. Kemp MG, Spandau DF, Simman R, and Travers JB. (2017). Insulin-like Growth Factor-1 Receptor Signaling is Required for Optimal ATR-CHK1 Kinase Signaling in Ultraviolet B (UVB)-irradiated Human Keratinocytes. J Biol Chem. 292(4): 1231-9. YouTube summary: click here.

 

University of North Carolina (Postdoctoral Research; 2006-2016)

  1. Canturk F, Karaman M, Selby CP, Kemp MG, Kulaksiz-Erkmen G, Hu J, Li W, Lindsey-Boltz LA, and Sancar A. (2016). Nucleotide excision repair by dual incisions in plants. Proc Natl Acad Sci USA.113(17): 4706-10.
  2. Kemp MG and Sancar A. (2016). ATR kinase inhibition protects non-cycling cells from the lethal effects of DNA damage and transcription stress. J Biol Chem. 291(17): 9330-42.
  3. Lindsey-Boltz LA, Kemp MG, Hu J, and Sancar A. (2015). Analysis of ribonucleotide removal from DNA by human nucleotide excision repair.  J Biol Chem. 290(50): 29801-7.
  4. Choi JH, Kim SY, Kim SK, Kemp MG, and Sancar A. (2015). An integrated approach for analysis of the DNA damage response in mammalian cells: Nucleotide excision repair, DNA damage checkpoints, and apoptosis. J Biol Chem. 290(48): 28812-21.
  5. Hao J, Li Y, de Renty C, Xiao H, Kemp MG, DePamphilis ML, and Zhu W (2015). And-1 coordinates with Claspin for efficient Chk1 activation in response to replication stress. EMBO J. 34(15): 2096-110.
  6. Kemp MG, Lindsey-Boltz LA, and Sancar A. (2015). UV light potentiates STING (stimulator of interferon genes)-dependent innate immune signaling through deregulation of ULK1 (Unc51-like kinase 1). J Biol Chem. 290(10): 12184-94.
  7. Gaddameedhi S, Selby CP, Kemp MG, Ye R, and Sancar A. (2015). The circadian clock controls sunburn apoptosis and erythema in mouse skin. J Invest Dermatology. 135(4): 1119-27.
  8. Lindsey-Boltz LA*, Kemp MG*, Capp C, and Sancar A. (2015). RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling. Cell Cycle. 14(1): 99-108. (*co-first author)
  9. Kemp MG, Gaddameedhi S, Choi JH, Hu J, and Sancar A. (2014). DNA repair synthesis and ligation affect the processing of excised oligonucleotides generated by human nucleotide excision repair. J Biol Chem. 289: 26574-26583.
  10. Choi JH, Gaddameedhi S, Kim SY, Hu J, Kemp MG, and Sancar A. (2014). Highly specific and sensitive method for measuring nucleotide excision repair kinetics of ultraviolet photoproducts in human cells. Nuc Acids Res. 42: e29
  11. Lindsey-Boltz LA, Kemp MG, Reardon JT, Derocco V, Iyer RR, Modrich P, and Sancar A. (2014). Coupling of human DNA excision repair and ATR-mediated DNA damage checkpoint in a define in vitro system. J Biol Chem. 289L 5074-5082.
  12. Hu J, Choi JH, Gaddameedhi S, Kemp MG, Reardon JT, and Sancar A. (2013). Nucleotide excision repair in human cells: Fate of the excised oligonucleotide carrying DNA damage in vivo. J Biol Chem. 288: 20918-20926.
  13. Hassan BH, Lindsey-Boltz LA, Kemp MG, and Sancar A. (2013). Direct role for the replication protein Treslin (Ticrr) in the ATR-mediated checkpoint response. J Biol Chem. 288: 18903-18910.
  14. Kemp MG, Reardon JT, Lindsey-Boltz LA, and Sancar A. (2012). Mechanism of release and fate of excised oligonucleotides during nucleotide excision repair. J Biol Chem. 287: 22889-99.
  15. Yilmaz S, Sancar A, and Kemp MG. (2011). Multiple ATR-Chk1 pathway proteins preferentially associate with checkpoint-inducing DNA substrates. PLoS One 6(7): e22986.
  16. Kemp MG, Lindsey-Boltz LA, and Sancar A. (2011). The DNA damage response kinases DNA-dependent protein kinase (DNA-PK) and Ataxia telangiectasia mutated (ATM) are stimulated by bulky adduct-containing DNA. J Biol Chem. 286: 19237-46.
  17. Sercin O and Kemp MG. (2011). Characterization of functional domains in human Claspin. Cell Cycle. 10(10): 1599-1606.
  18. Choi JH, Lindsey-Boltz LA, Kemp M, Mason AC, Wold MS, and Sancar A. (2010). Reconstitution of RPA-covered single-stranded DNA-activated ATR-Chk1 signaling. Proc Natl Acad Sci USA. 107(31): 13660-5.
  19. Gaddameedhi S, Kemp MG, Shields JM, Reardon JT, Smith-Roe SL, Kaufmann WK, and Sancar A. (2010). Nucleotide excision repair capacity of melanocytes and melanoma cell lines. Cancer Res. 70(12): 4922-30.
  20. Kemp MG, Akan Z, Yilmaz S, Grillo M, Smith-Roe SL, Kang TH, Cordeiro-Stone M, Kaufmann WK, Abraham RT, Sancar A, and Unsal-Kacmaz K. (2010). Tipin-RPA interaction mediates Chk1 phosphorylation by ATR in response to genotoxic stress. J Biol Chem. 285(22): 16562-71.
  21. Kemp MG, Mason AC, Carreira A, Reardon JT, Haring SJ, Borgstahl GE, Kowalczykowski SC, Sancar A, and Wold MS. (2010). An alternative form of replication protein A expressed in normal human tissues supports DNA repair. J Biol Chem. 285(7): 4788-97.
  22. Kang TH, Reardon JT, Kemp M, and Sancar A. (2009). Circadian oscillation of nucleotide excision repair in mammalian brain. Proc Natl Acad Sci USA. 106(8):2864-73.

Wright State University (Doctoral Research; 2001-2006)

  1. Chowdhury A, Liu G, Kemp M, Chen X, Katrangi N, Myers S, Ghosh M, Yao J, Gao Y, Bubulya P, Leffak M. (2010). The DNA unwinding element binding protein DUE-B interacts with Cdc45 in preinitiation complex formation. Mol Cell Biol. 30(6): 1495-507.
  2. Kemp M, Bae B, Yu JP, Ghosh M, Leffak M, and Nair SK. (2007). Structure and function of the c-myc DNA-unwinding element-binding protein DUE-B. J Biol Chem. 282(14): 10441-8.
  3. Ghosh M, Kemp M, Liu G, Ritzi M, Schepers A, and Leffak M. (2006). Differential binding of replication proteins across the c-myc replicator. Mol Cell Biol. 26(14): 5270-83.
  4. Casper JM*, Kemp MG*, Ghosh M, Randall GM, Vaillant A, and Leffak M. (2005). The c-myc DNA-unwinding element-binding protein modulates the assembly of DNA replication complexes in vitro. J Biol Chem. 280(13): 13071-83. (*co-first author)
  5. Kemp MG, Ghosh M, Liu G, and Leffak M. (2005). The histone deacetylase inhibitor trichostatin A alters the pattern of DNA replication origin activity in human cells. Nuc Acids Res. 33(1): 325-336.

 

Invited Reviews/Book Chapters/Commentaries:

  1. Ume AC, Pugh JM, Kemp MG, Williams CR. (2020). Calcineurin inhibitor (CNI)-associated skin cancers: New insights on exploring mechanisms by which CNIs downregulate DNA repair machinery. Photoderm Photoimmunol Photomed. 36(6): 433-440.
  2. Gabbard RD, Hoopes RR, and Kemp MG. (2020). Spironolactone and XPB: An Old Drug with a New Molecular Target. Biomolecules. 10(5): 756.
  3. Kemp MG. (2019). Damage removal and gap filling in nucleotide excision repair. The Enzymes: DNA Repair, 45: 59-97.
  4. Khan AQ, Travers JB, and Kemp MG. (2018). Roles of UVA Radiation and DNA Damage Responses in Melanoma Pathogenesis. Environmental and Molecular Mutagenesis, 59(5): 438-60.
  5. Kemp MG, Spandau DF, and Travers JB. (2017). Impact of Age and Insulin-like Growth Factor-1 on DNA Damage Responses in UV-irradiated Human Skin. Molecules. 22(3).
  6. Kemp MG. (2017). Crosstalk Between Apoptosis and Autophagy: Environmental Genotoxins, Infection, and Innate Immunity. J Cell Death. 1-6.
  7. Kemp MG and Hu J. (2017). Post-Excision Events in Human Nucleotide Excision Repair. Photochemistry & Photobiology. 93(1): 178-91.
  8. Song J, Kemp MG and Choi J-H. (2017). Detection of the Excised, Damage-containing Oligonucleotide Products of Nucleotide Excision Repair in Human Cells. Photochemistry & Photobiology. 93(1): 192-8.
  9. Sancar A, Linsdey-Boltz LA, Gaddameedhi S, Selby CP, Ye R, Chiou YY, Kemp MG, Hu J, Lee SH, and Ozturk N. (2015). Circadian Clock, Cancer, and Chemotherapy. Biochemistry. 54(2): 110-23.
  10. Kemp MG and Sancar A. (2012). DNA excision repair: Where do all the dimers go? Cell Cycle. 11(16): 2997-3001.
  11. Kemp MG and Sancar A. (2009). DNA distress: just ring 9-1-1. Current Biology. 19(17): R2864-7.

 

Other Writing:

  1. Kemp MG. (2015). NextGen’s tools for the future. Science. 347(6230): 34.
  2. Kemp MG. (2015). NextGen’s course catalog. Science. 347(6217): 24.
  3. Kemp MG. (2014). NextGen Voices: Science advocacy. Science. 344(6179): 37.

Professional Affiliations/Memberships

American Society for Biochemistry and Molecular Biology (ASBMB), 2015-present

Environmental Mutagenesis and Genomics Society (EMGS), 2016-present

American Society for Photobiology (ASP), 2016-present

Society for Investigative Dermatology (SID), 2017-present

Awards/Recognition

May 2019        Newly Independent Investigators Engagement Program, Environmental Mutagenesis and Genomics Society

Mar 2018         Travel Award, Society for Investigative Dermatology

Sept 2016        Emerging Scientist Travel Award, Environmental Mutagenesis and Genomic Society

May 2016        1st Place, Platform Presentation, 18th Annual Midwest DNA Repair Symposium

May 2011        1st Place, Platform Presentation, 13th Annual Midwest DNA Repair Symposium

May 2010        1st Place, Platform Presentation, 12th Annual Midwest DNA Repair Symposium

Dec 2006         NIH-NRSA Institutional Postdoctoral Training Fellowship, Lineberger Comprehensive Cancer Center, University of North Carolina

Sept 2003        Oral Presentation Award, Wright State University Molecular Biology Retreat

May 2003        Student Excellence Award, Wright State University Biomedical Sciences Ph.D. Program

Aug 2002         Sigma Xi Student Membership Award, Wright State University

June 1999       Cum laude Graduation Honors, Wright State University                                                       

Apr 1995         Charles H. Hewitt Memorial Scholarship, Wright State University

Mar 1999         McDonald’s Scholarship Recipient

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